Guide to Mutation Detection

Download or read book Guide to Mutation Detection written by Human Genome Organisation and published by John Wiley & Sons. This book was released on 2005-02-03 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to both the theory and practice of mutation detection technology, including detailed practical protocols that are derived from courses run by the Human Genome Organization (HUGO). Guide to Mutation Detection offers the only single source for assessing the molecular biological tools for mutation analysis in order to design an experiment. The first section reviews topics such as key technologies, mutation scanning, cleavage, quality control, high throughput approaches, databases, and nomenclature. The second part covers step-by-step protocols and discussion of methods such as tag-array minisequencing, electronic hybridization, pyrosequencing, fluorescent SSCP, DHPLC, array CGH, and MADGE.