Towards Multi-colour Strategies for the Detection of Nucleic Acid Hybridization Using Quantum Dots as Energy Donors in Fluorescence Resonance Energy Transfer (FRET).
Author | : Walter Russell Algar |
Publisher | : |
Total Pages | : 360 |
Release | : 2006 |
ISBN-10 | : 0494210419 |
ISBN-13 | : 9780494210413 |
Rating | : 4/5 (413 Downloads) |
Download or read book Towards Multi-colour Strategies for the Detection of Nucleic Acid Hybridization Using Quantum Dots as Energy Donors in Fluorescence Resonance Energy Transfer (FRET). written by Walter Russell Algar and published by . This book was released on 2006 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The unique optical properties of quantum dots (QDs) are of interest in the development of nucleic acid diagnostics. The potential for a simultaneous two-colour diagnostic scheme for nucleic acids operating on the basis of fluorescence resonance energy transfer (FRET) has been demonstrated. Upon ultraviolet excitation, two-colours of CdSe/ZnS quantum dots with conjugated oligonucleotide probes acted as energy donors yielding FRET-sensitized acceptor emission upon hybridization with fluorophore labeled target oligonucleotides. The use of an intercalating dye to improve signal-to-noise was also demonstrated. The major limitation of the system was the non-specific adsorption of oligonucleotides, which was characterized extensively. Adsorptive interactions were found to affect the conformation of oligonucleotides conjugated to QDs, the kinetics of hybridization with QD-DNA conjugates, and the thermal stability of those hybrids. In addition, it was found that thiol-alkyl-acid capped QDs exhibited pKa correlated ligand-chromism and radiative decay rate-driven changes in quantum yield.